PROCHECK-NMR - Sample plots

3. Chi1-Chi2 plots

Description

The Chi1-Chi2 plots show the chi1-chi2 torsion angle combinations for all residue types that have both these angles.

The shading on each plot indicates how favourable each region on the plot is; the darker the shade the more favourable the region. The data on which the shading is based has come from a data set of 163 non-homologous, high-resolution protein chains chosen from structures solved by X-ray crystallography to a resolution of 2.0Å or better and an R-factor no greater than 20%.

The numbers in brackets, following each residue name, show the total number of data points on that graph. The numbers within each data point correspond to the model number, while any red numbers above the data points are the reside-numbers of the residues in question (ie showing those residues lying in unfavourable regions of the plot).

Options

The main options for the Chi1-Chi2 plots are:-

• The cut-off G-factor defining which points are to be labelled as lying in unfavourable regions of the plot.
• The plot can be in colour or black-and-white.

These options can be altered by editing the parameter file, procheck_nmr.prm, as described here.

PROCHECK-NMR - Sample plots